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Evidence for Extensive Distribution of Oestrogen Receptor αImmunoreactivity in the Cerebral Cortex of Adult Rats
Author(s) -
Jonathan Butler,
Imre Kalló,
Maria Sjöberg,
Clive W. Coen
Publication year - 1999
Publication title -
journal of neuroendocrinology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.062
H-Index - 116
eISSN - 1365-2826
pISSN - 0953-8194
DOI - 10.1046/j.1365-2826.1999.00346.x
Subject(s) - paraformaldehyde , immunohistochemistry , medicine , endocrinology , cerebral cortex , cingulate cortex , immunoprecipitation , chemistry , cortex (anatomy) , antibody , biology , neuroscience , central nervous system , immunology , organic chemistry
The presence of oestrogen receptor alpha (ER α )‐immunoreactivity (IR) in the cerebral cortex of adult rats has been investigated. Previous studies have reported a negligible presence of ER α or ER α mRNA in this region of the adult rat brain. We have used immunoprecipitation and immunohistochemistry, with various antibodies and fixatives, to detect this protein in the cingulate cortex. When the tissue was fixed using paraformaldehyde alone only faint ER α –IR was observed at this site. In contrast, following fixation either with acrolein (with or without paraformaldehyde) or with a mixture of paraformaldehyde and glutaraldehyde there was extensive ER α –IR throughout layers II to VI; this was absent when the antibodies were preincubated with the peptide fragment used in their production. The presence of ER α –IR in the nonfixed cingulate cortex of adult rats was confirmed by immunoprecipitation.