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Rapid and Sensitive Vasopressin Heteronuclear RNA Responses to Changes in Plasma Osmolality
Author(s) -
Hiroshi Arima,
Kunïkazu Kondo,
Satoshi Kakiya,
Hiroshi Nagasaki,
Hisashi Yokoi,
Yuko Yambe,
Takashi Murase,
Yoshinobu Iwasaki,
Yutaka Oiso
Publication year - 1999
Publication title -
journal of neuroendocrinology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.062
H-Index - 116
eISSN - 1365-2826
pISSN - 0953-8194
DOI - 10.1046/j.1365-2826.1999.00308.x
Subject(s) - vasopressin , endocrinology , medicine , plasma osmolality , hypertonic saline , supraoptic nucleus , osmole , chemistry , saline , osmoregulation , tonicity , biology , ecology , salinity
The plasma concentration of arginine vasopressin (AVP) is closely regulated by plasma osmolality. In this study, we used intronic in‐situ hybridization to investigate the transcriptional activity of the AVP gene in the supraoptic nucleus (SON) and paraventricular nucleus (PVN) after intraperitoneal (i.p.) injection of hypertonic saline inducing small changes in plasma osmolality in rats. After ip injection of 900 mOsm/kg saline (2% BW), plasma [Na] reached the highest level at 10 min (900 mOsm/kg, 146±1 mEq/l; 290 mOsm/kg, 139±1 mEq/l, P<0.01) and maintained that level until 30 min. The expression of AVP heteronuclear (hn) RNA in the SON and PVN increased significantly as early as 10 min and peaked 30 min after ip injection of the hypertonic saline. AVP hnRNA expression showed a significant increase even after a 2 mEq/L elevation in plasma [Na] concentration, and increased dose‐dependently in response to the concentration of saline injection (290–900 mOsm/kg). These results demonstrate that, similar to AVP secretion, AVP gene transcriptional activity in the SON and PVN is closely regulated by plasma osmolality.