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Anorectic Effect of Pituitary Adenylate Cyclase Activating Polypeptide (PACAP) in Rats: Lack of Evidence for Involvement of Hypothalamic Neuropeptide Gene Expression
Author(s) -
Mizuno Yumiko,
Kondo Kunikazu,
Terashima Yasuhiro,
Arima Hiroshi,
Murase Takashi,
Oiso Yutaka
Publication year - 1998
Publication title -
journal of neuroendocrinology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.062
H-Index - 116
eISSN - 1365-2826
pISSN - 0953-8194
DOI - 10.1046/j.1365-2826.1998.00244.x
Subject(s) - medicine , endocrinology , galanin , neuropeptide , anorectic , neuropeptide y receptor , hypothalamus , corticotropin releasing hormone , in situ hybridization , pituitary adenylate cyclase activating peptide , chemistry , cyclase , adenylate kinase , biology , vasoactive intestinal peptide , messenger rna , receptor , gene , food intake , stimulation , biochemistry
We investigated the effect of centrally administered pituitary adenylate cyclase activating polypeptide (PACAP) on feeding in rats, and the involvement of hypothalamic neuropeptide gene expression using in situ hybridization. lntracerebroventricular injection of PACAP (1000 pmol/rat) significantly decreased food intake in a dose‐dependent manner. In PACAP‐treated rats, neuropeptide Y (NPY) mRNA levels in the arcuate nucleus and galanin mRNA levels in the paraventricular nucleus increased, and corticotropin‐releasing hormone (CRH) mRNA levels in the paraventricular nucleus decreased. In rats fasted for 72 h, NPY mRNA levels increased, and CRH mRNA levels decreased, but galanin mRNA levels were unchanged. These results indicate that the anorectic function of PACAP is not mediated by NPY or CRH, and that PACAP increases galanin synthesis.