Nitric Oxide Mediates Gonadotropin‐Releasing Hormne Effects on Frog Pituitary

We studied the possible role of nitric oxide (NO) in GnRH‐induced gonadotropin secretion in the female water frog, Rana esculenta. During pre‐reproduction, pre‐ovulation, ovulation, post‐ovulation, refractory, recovery and hibernation, pituitaries were incubated with medium‐alone, GnRH, NO donor (NOd), NO synthase inhibitor (NOSi), cyclic GMP analogue (cGMPa), soluble guanylate cyclase inhibitor (sGCi), GnRH plus NOSi, GnRH plus sGCi, and NOd plus sGCi. Because antisera raised against gonadotropins are not available for this species, we measured these hormones indirecly through their effects on ovarian progesterone secretion. The ovaries were superfused with the pituitaries pre‐incubated as reported above. In addition, NOS activity and cGMP levels were determined in the pre‐incubated pituitaries. Those pre‐incubated with medium‐alone and with GnRH increased progesterone secretion during pre‐reproduction, pre‐ovulation, ovulation and recovery; the increase induced by GnRH was higher than that induced by medium‐alone during pre‐reproduction, pre‐ovulation and recovery. NOd and cGMPa increased progesterone in all considered reproductive phases except ovulation; the increase induced by NOd and cGMP was higher than that induced by medium‐alone during pre‐reproduction, pre‐ovulation and recovery. NOS activity was highest during ovulation and lowest during post‐ovulation, refractory and hibernation. GnRH increased NOS activity during pre‐reproduction, pre‐ovulation and recovery. Cyclic GMP levels were highest during ovulation and lowest during post‐ovulation, refractory and hibernation. GnRH increased cGMP levels during pre‐reproduction, pre‐ovulation and recovery, NOd during all considered reproductive phases. These results suggest that NO mediates basal and GnRH‐induced gonadotropin secretion in female Rana esculenta .