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Corticosteroid Effects on Gene Expression of Myelin Basic Protein in Oligodendrocytes and of Glial Fibrillary Acidic Protein in Type 1 Astrocytes
Author(s) -
Melcangi R.C.,
Magnaghi V.,
Cavarretta I.,
Riva M.A.,
Martini L.
Publication year - 1997
Publication title -
journal of neuroendocrinology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.062
H-Index - 116
eISSN - 1365-2826
pISSN - 0953-8194
DOI - 10.1046/j.1365-2826.1997.00621.x
Subject(s) - glial fibrillary acidic protein , myelin basic protein , endocrinology , medicine , astrocyte , corticosterone , oligodendrocyte , biology , myelin , gfap stain , neuroglia , mineralocorticoid , gene expression , glucocorticoid , hormone , central nervous system , gene , immunohistochemistry , biochemistry
The paper describes the effects of corticosterone and deoxycorticosterone (DOC), used in their native or in their 5 & agr;‐reduced molecular forms (dihydrocorticosterone, DHC; dihydrodeoxycorticosterone, DHDOC; and tetrahydrodeoxycorticosterone, THDOC) on the gene expression of the myelin basic protein (MBP) and of the glial fibrillary acidic protein (GFAP) in pure cultures, respectively, of oligodendrocytes and type 1 astrocytes obtained from the neonatal rat brain. Among the different steroids tested (corticosterone, DHC, DOC, DHDOC and THDOC), only DHDOC was effective on the gene expression of MBP in the oligodendrocyte cultures; the mRNA levels of this typical oligodendrocyte marker were decreased following exposure to this steroid for 24 h. In the case of the astrocytic marker GFAP, its gene expression was increased by the exposure to corticosterone for 6 and 24 h, while DHC was ineffective; the mineralocorticoid DOC was also ineffective, while its 5 & agr;‐reduced derivative, DHDOC, strongly inhibited GFAP gene expression, starting at 6 h after beginning of the treatment.