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Reducing image distortions due to temperature‐related microscope stage drift
Author(s) -
Adler J.,
Pagakis S. N.
Publication year - 2003
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1046/j.1365-2818.2003.01160.x
Subject(s) - microscope , stage (stratigraphy) , confocal , optics , materials science , optical microscope , enclosure , computer vision , physics , computer science , scanning electron microscope , geology , paleontology , telecommunications
Summary Distortions in confocal 3D image data sets were related to movements of the microscope stage that matched fluctuations in laboratory temperature. Movement was apparent in all three axes. Adding a draught‐proof enclosure, covering the stage, objective lenses and supporting structures, minimized these short‐term fluctuations. However, the stage still tracked slow changes in laboratory temperature and was sensitive to heat sources mounted on the microscope. Suggestions are made about microscope design.