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Efficient embedding technique for preparing small specimens for stereological volume estimation: zebrafish larvae
Author(s) -
Hill A. J.,
Howard C. V.,
Cossins A. R.
Publication year - 2002
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1046/j.1365-2818.2002.01025.x
Subject(s) - stereology , embedding , volume (thermodynamics) , sample (material) , sampling (signal processing) , larva , biological system , materials science , biology , computer science , biomedical engineering , anatomy , chemistry , physics , artificial intelligence , chromatography , computer vision , ecology , medicine , filter (signal processing) , quantum mechanics , endocrinology
Summary Stereological sampling regimes, in particular volume and number estimation, often require systematic uniformly random sections throughout a specimen. A method has been developed to increase the efficiency of preparing fish larvae for sectioning prior to histological or stereological analysis. Embedding a group of larvae in a resin block using this technique greatly reduces the quantity of sections produced and allows easy assessment of sample groups. Saving time in this way therefore makes stereology a more viable research tool.