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Thickness measurements of lacey carbon films
Author(s) -
Karlsson G.
Publication year - 2001
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1046/j.1365-2818.2001.00930.x
Subject(s) - materials science , carbon fibers , layer (electronics) , composite material , polymer , carbon film , nanotechnology , thin film , composite number
Lacey carbon filmed grids interact differently with liquid samples during plunging and subsequent studies in cryo‐TEM depending on type and manufacture. One important parameter is the thickness of the film, which is investigated here by ultrathin sectioning. The film thickness of the carbon‐Formvar type is found to be 100–200 nm, with the variation of the thickness occurring in the polymer layer. The pure carbon type is found to be 20–30 nm thick. Knowing the thickness makes it easier to choose the optimal type for each cryo‐TEM sample.