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Observation of human dentine by focused ion beam and energy‐filtering transmission electron microscopy
Author(s) -
Hoshi K.,
Ejiri S.,
Probst W.,
Seybold V.,
Kamino T.,
Yaguchi T.,
Yamahira N.,
Ozawa H.
Publication year - 2001
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1046/j.1365-2818.2001.00784.x
Subject(s) - transmission electron microscopy , focused ion beam , ion , materials science , microscopy , transmission (telecommunications) , energy (signal processing) , energy filtered transmission electron microscopy , electron microscope , ion beam , optics , scanning transmission electron microscopy , chemistry , beam (structure) , nanotechnology , computer science , physics , telecommunications , organic chemistry , quantum mechanics
Molar dentine was sliced into 100 nm ultrathin sections, by means of a focused ion beam, for observation by energy‐filtering transmission electron microscopy (EFTEM). Within the matrix, crystals approximately 10 nm wide and 50–100 nm long were clearly observed. When carbon and calcium were mapped in electron spectroscopic images by EFTEM, carbon failed to localize in crystals. However, it was found in other regions, especially those adjacent to crystals. Because carbon localizations were thought to reflect the presence of organic components, carbon concentration in regions near crystals suggested the interaction of crystals and organics, leading to organic control of apatite formation and growth. Ca was present in almost all regions. The majority of Ca localizing in regions other than crystals may be bound to organic substances present in dentine matrix. These substances are thought to both accumulate Ca and act as reservoirs for crystallization of apatite in dentine.