Premium
Penetration pathways of fluorescent dyes in human hair fibres investigated by scanning near‐field optical microscopy
Author(s) -
Kelch A.,
Wessel S.,
Will T.,
Hintze U.,
Wepf R.,
Wiesendanger R.
Publication year - 2000
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1046/j.1365-2818.2000.00756.x
Subject(s) - penetration (warfare) , fluorescence , microscopy , materials science , optical microscope , fluorescence microscope , optics , biophysics , scanning electron microscope , chemistry , biology , composite material , physics , operations research , engineering
Thin cross‐sections of human hairs were investigated by scanning near‐field optical microscopy (SNOM) and confocal laser scanning microscopy (CLSM) after penetration of a fluorescent dye. The same samples were measured with both techniques to compare the observed structures. The images obtained from the two methods show nearly identical structures representing pathways of the dye molecules in hairs. The SNOM images provide a higher resolution than the CLSM images. Therefore, SNOM is believed to be a suitable method for investigations at a resolution of 100 nm on penetration pathways of fluorescent dyes such as the cell membrane complex pathway in cross‐sections of hairs.