Premium
ER‐Tracker dye and BODIPY‐brefeldin A differentiate the endoplasmic reticulum and Golgi bodies from the tubular‐vacuole system in living hyphae of Pisolithus tinctorius
Author(s) -
Louise Cole,
Danielle Davies,
Geoffrey J. Hyde,
A. E. Ashford
Publication year - 2000
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1046/j.1365-2818.2000.00664.x
Subject(s) - brefeldin a , endoplasmic reticulum , golgi apparatus , vacuole , hypha , microbiology and biotechnology , biology , biophysics , chemistry , botany , cytoplasm
Two fluorochromes, ER‐Tracker TM Blue‐White DPX dye and the fluorescent brefeldin A (BFA) derivative, BODIPY‐BFA, label the endoplasmic reticulum (ER) in hyphal tips of Pisolithus tinctorius and allow its differentiation from the tubular‐vacuole system at the light microscope level in living cells. The ER‐Tracker dye labels a reticulate network similar in distribution to ER as seen in electron micrographs of freeze‐substituted hyphae. BODIPY‐BFA stains a thicker axially aligned structure with an expanded region at the apex, which is similar to that seen when hyphae are stained with ER‐Tracker dye in the presence of unconjugated BFA. This structure is considered to be ER modified by BFA, a view supported by ultrastructural observations of the effect of BFA on the fungal ER. Both fluorescent probes also stain punctate structures, which are most likely to be Golgi bodies. Neither probe labels the tubular‐vacuole system.