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Soft X‐ray microscopy with a cryo scanning transmission X‐ray microscope: I. Instrumentation, imaging and spectroscopy
Author(s) -
J. Mäser,
A. Osanna,
Y Wang,
Chris Jacobsen,
Janos Kirz,
S. Spector,
Barry Winn,
D. M. Tennant
Publication year - 2000
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1046/j.1365-2818.2000.00630.x
Subject(s) - microscope , microscopy , synchrotron radiation , materials science , x ray , synchrotron , optics , spectroscopy , optical microscope , instrumentation (computer programming) , scanning electron microscope , physics , quantum mechanics , computer science , operating system
We have developed a cryo scanning transmission X‐ray microscope which uses soft X‐rays from the National Synchrotron Light Source. The system is capable of imaging frozen hydrated specimens with a thickness of up to 10 μm at temperatures of around 100 K. We show images and spectra from frozen hydrated eukaryotic cells, and a demonstration that biological specimens do not suffer mass loss or morphological changes at radiation doses up to about 10 10 Gray. This makes possible studies where multiple images of the same specimen area are needed, such as tomography (Wang et al. (2000) Soft X‐ray microscopy with a cryo scanning transmission X‐ray microscope: II. Tomography. J. Microsc . 197, 80–93) or spectroscopic analysis.