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Flat‐bed scanning as a tool for quantitative neuroimaging
Author(s) -
Oliver Schmitt,
Reinhard Eggers
Publication year - 1999
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1046/j.1365-2818.1999.00604.x
Subject(s) - laminar flow , scanner , cresyl violet , materials science , neuroimaging , biomedical engineering , optics , cerebral cortex , high resolution , geology , physics , neuroscience , biology , medicine , remote sensing , staining , pathology , thermodynamics
The aim of this study was to compare three different imaging techniques which are used to provide data on the laminar structure of the human cerebral cortex. Region V1 of Brodmann's area 17 stained with cresyl violet was investigated, and a conventional semi‐automatic morphometric evaluation, the videomicroscropic procedure and a new transparent flat‐bed scanning technique were compared. The results of each digitizing method were converted into normalized profiles which allow the laminae in the striate cortex to be displayed. It was found that major laminar patterns can be detected by the scanning technique, but that subsidiary laminations are more clearly displayed by morphometry and videomicroscopy. For magnifications up to × 400 a high resolution transparent flat‐bed scanner may be used in place of the videomicroscopy technique.