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Cryo‐techniques applied to stratum corneum with description of a new sample holder for cryo‐scanning electron microscopy of freeze‐fractured samples
Author(s) -
Hallegot,
Mido
Publication year - 1999
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1046/j.1365-2818.1999.00586.x
Subject(s) - stratum corneum , scanning electron microscope , stratum , microscopy , sample preparation , electron microscope , sample (material) , cryo electron microscopy , materials science , chemistry , biophysics , optics , chromatography , pathology , biology , physics , medicine , composite material , paleontology
Stratum corneum structure greatly differs from that of the living epidermis and specific sample cryo‐preparation techniques have to be used. Practical aspects of these cryo‐techniques applied to stratum corneum are discussed. Emphasis is placed on scanning electron microscopy of cryo‐fixed samples. A new sample holder designed for cryo‐scanning electron microscopy of freeze‐fractured stratum corneum is described.