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Real‐time imaging of two‐photon‐induced fluorescence with a microlens‐array scanner and a regenerative amplifier
Author(s) -
Fujita K.,
Nakamura O.,
Kaneko T.,
Kawata S.,
Oyamada M.,
Takamatsu T.
Publication year - 1999
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1046/j.1365-2818.1999.00493.x
Subject(s) - microlens , amplifier , optics , scanner , microscope , materials science , two photon excitation microscopy , fluorescence , microscopy , fluorescence microscope , laser , fluorescence lifetime imaging microscopy , optoelectronics , physics , lens (geology) , cmos
A real‐time two‐photon fluorescence microscope with a microlens‐array scanner using a regenerative amplifier is presented. The regenerative amplifier generates pulsed laser lights with extremely high peak power and produces brighter two‐photon‐induced fluorescence compared with that produced without the amplifier. Experimental results obtained from the observation of some biological samples with the proposed microscope are given.