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Rapid staining of ultrathin sections with the use of a microwave oven
Author(s) -
İlkin Çavuşoğlu,
Zeynep Kahveci,
S A Sirmali
Publication year - 1998
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1046/j.1365-2818.1998.00415.x
Subject(s) - uranyl acetate , microwave oven , staining , microwave , microwave irradiation , fixation (population genetics) , electron microscope , chemistry , electron micrographs , materials science , biomedical engineering , optics , pathology , biochemistry , computer science , physics , medicine , telecommunications , gene
The microwave oven has many potential applications, ranging from tissue fixation to staining for light and electron microscopy. This study was planned to speed up the staining of ultrathin sections. The first set of grids was stained conventionally with uranyl acetate and Reynold's lead citrate solutions. The other grids were stained with the same solutions by microwave irradiation. The electron micrographs of grids stained using the microwave technique were as satisfactory as the grids stained conventionally. Microwave‐treated grids demonstrated more uniform staining and less precipitate. The use of a microwave oven shortened staining time by ≈ 38 min.