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Improved staining of F‐actin and co‐localization of mitochondria in plant cells
Author(s) -
Olyslaegers G.,
Verbelen J. P.
Publication year - 1998
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1046/j.1365-2818.1998.00398.x
Subject(s) - organelle , phalloidin , actin , mitochondrion , membrane , chemistry , staining , biophysics , cell , microbiology and biotechnology , glycerol , biochemistry , cytoskeleton , biology , genetics
Permeating the cell membrane with glycerol instead of detergents (e.g. NP‐40) and/or organic solvents (e.g. DMSO) significantly improves the visualization of F‐actin with rhodamine phalloidin. In particular, the thinnest bundles are more intensely stained, better delineated and observation time is prolonged dramatically. Furthermore, the method is gentle to membranous organelles, which makes, for example, co‐localization of mitochondria and actin possible.