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Towards automatic cell identification in DIC microscopy
Author(s) -
David Young,
C. A. Glasbey,
Alison Gray,
Nadine Martin
Publication year - 1998
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1046/j.1365-2818.1998.00397.x
Subject(s) - identification (biology) , microscopy , computer science , computational biology , nanotechnology , materials science , biology , optics , physics , botany
A general method is proposed for constructing templates of cells in differential interference contrast (DIC) microscopy. This takes account of the optics which generate DIC images, and is applicable to both transparent and semi‐transparent cells of simple and complex shapes. Then, a template matching methodology is presented, which uses fast Fourier transforms to fit templates of a range of sizes and orientations to images. For illustration, this is used to automatically identify and measure individual Candida yeast cells in clusters.