Premium
Electron energy‐loss spectroscopy (EELS) of iodine in thyroxine: parameters of radiation‐induced loss of iodine
Author(s) -
Haking,
; Richter,
TRÖster,
Spring,
Richard Spiess,
Trendelenburg
Publication year - 1998
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1046/j.1365-2818.1998.00287.x
Subject(s) - iodine , irradiation , electron energy loss spectroscopy , chemistry , spectroscopy , electron , radiochemistry , radiation , iodine compounds , radiation damage , analytical chemistry (journal) , materials science , transmission electron microscopy , nanotechnology , optics , environmental chemistry , organic chemistry , physics , quantum mechanics , nuclear physics
The loss of iodine during irradiation with electrons was measured in order to determine whether it can be detected by electron spectroscopic imaging (ESI). Since iodine can be bound to organic molecules, it could be a candidate for tracer studies by ESI in biomedical research. A solution of thyroxine spread over a carbon film was used as a test specimen. Thyroxine contains four iodine atoms covalently bound to aromatic rings. For ESI, higher doses have to be used than in conventional electron microscopy. The iodine content was therefore measured after irradiation with doses of up to 3 × 10 7 el nm −2 . The measurements were carried out for different specimen thicknesses, temperatures and dose rates, and it was found that iodine can be detected with ESI if moderate dose rates are used at a temperature of −160 °C.