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Highly efficient upconverters for multiphoton fluorescence microscopy
Author(s) -
Wood-Hi Cheng,
Pan Jian,
Shih,
Ki Wook Kim,
Liou,
HaeSim Park
Publication year - 1998
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1046/j.1365-2818.1998.00277.x
Subject(s) - two photon excitation microscopy , microscopy , fluorescence , fluorescence microscope , materials science , laser , absorption (acoustics) , optics , two photon absorption , super resolution microscopy , multiphoton fluorescence microscope , excitation , resolution (logic) , photon , microscope , absorption cross section , optoelectronics , cross section (physics) , physics , computer science , quantum mechanics , artificial intelligence
A new generation of efficient two‐photon absorbing fluorescent molecules has been developed and used effectively for two‐photon laser scanning microscopy. Several examples of the use of these new fluorophores have been presented. In addition, issues relating to the two‐photon absorption cross‐section, excitation power, sample properties and resolution in two‐photon laser scanning microscopy are discussed.