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Far‐field fluorescence microscopy with three‐dimensional resolution in the 100‐nm range
Author(s) -
Hell S. W.,
Schrader M.,
Van Der Voort H. T. M.
Publication year - 1997
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1046/j.1365-2818.1997.2410797.x
Subject(s) - microscopy , confocal microscopy , fluorescence microscope , two photon excitation microscopy , resolution (logic) , confocal , optics , light sheet fluorescence microscopy , fluorescence , lambda , super resolution microscopy , photoactivated localization microscopy , materials science , isotropy , physics , artificial intelligence , computer science
We report three‐dimensional (3D) microscopy with nearly isotropic resolution in the λ/5 − λ/10 range. Our approach combines 4Pi‐confocal two‐photon fluorescence microscopy with image restoration. The 3D resolution is demonstrated with densely clustered beads as well as with F‐actin fibers in mouse fibroblast cells. A comparison with unrestored two‐photon confocal images reveals a total reduction of the uncertainty volume up to a factor of 15.