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Quantitative image analysis of birefringent biological material
Author(s) -
Ross S.,
Newton R.,
Zhou Y.M.,
Haffegee J.,
Ho M.W.,
Bolton J. P.,
Knight D.
Publication year - 1997
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1046/j.1365-2818.1997.2160776.x
Subject(s) - birefringence , liquid crystal , polarized light microscopy , phase contrast microscopy , optics , optical microscope , contrast (vision) , materials science , image contrast , phase (matter) , microscopy , intensity (physics) , interference (communication) , chemistry , physics , computer science , scanning electron microscope , computer network , channel (broadcasting) , organic chemistry
A previously described optical technique for enhancing interference colour contrast in polarized light microscopy is adapted for quantitative image analysis. This is based on a linear relationship for colour intensity vs. molecular birefringence and degree of phase alignment, derived using nematic liquid crystal approximations, and verified experimentally. The image analysis is described and illustrated on a section of the marginal rib of the dogfish egg case.