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Methods for X‐ray microanalysis of epidermis: the effect of local anaesthesia
Author(s) -
GRÄNGSJÖ A.,
LINDBERG M.,
ROOMANS G. M.
Publication year - 1997
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1046/j.1365-2818.1997.00195.x
Subject(s) - lidocaine , stratum spinosum , microanalysis , epidermis (zoology) , local anaesthetic , chemistry , local anesthesia , electron probe microanalysis , anesthesia , biopsy , general anaesthesia , stratum corneum , medicine , pathology , anatomy , mineralogy , electron microprobe , organic chemistry
The effect of local anaesthesia on the elemental content of cells in human epidermis was studied by electron probe X‐ray microanalysis. Local anaesthesia with lidocaine was given by intracutaneous injection within 1 min prior to taking a skin biopsy. Biopsies taken without local anaesthesia were used as controls. Lidocaine with or without adrenaline caused a significant increase in the concentrations of Na and Cl, and a decrease in the concentration of K in the cells of the stratum basale and the stratum spinosum, compared with the control samples. The presence of adrenalin in the anaesthetic did not change the effect of lidocaine. The effects of local anaesthesia have to be considered in planning and interpretation of clinical applications of X‐ray microanalysis.