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Real‐time two‐photon confocal microscopy using a femtosecond, amplified Ti:sapphire system
Author(s) -
BRAKENHOFF G. J.,
SQUIER J.,
NORRIS T.,
BLITON A. C.,
WADE M. H.,
ATHEY B.
Publication year - 1996
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1046/j.1365-2818.1996.97379.x
Subject(s) - confocal , femtosecond , confocal microscopy , optics , sapphire , materials science , two photon excitation microscopy , microscopy , biological imaging , photon , microscope , optoelectronics , laser , physics , fluorescence
The bilateral imaging approach known from confocal applications operating in the line mode was used to realize real‐time two‐photon imaging. It is shown that the sectioning inherent to two‐photon imaging could be improved by the introduction of a confocal line aperture in the imaging path. Using a high‐power, low‐repetition‐rate amplified Ti:sapphire system, various biological objects were visualized including live boar sperm.