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Application of confocal scanning laser microscopy for an automated nuclear grading of prostate lesions in three dimensions
Author(s) -
BEIL M.,
IRINOPOULOU T.,
VASSY J.,
RIGAUT J. P.
Publication year - 1996
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1046/j.1365-2818.1996.920650.x
Subject(s) - confocal , grading (engineering) , prostate , confocal microscopy , homogeneous , microscopy , artificial intelligence , confocal laser scanning microscopy , computer science , pattern recognition (psychology) , materials science , pathology , biomedical engineering , optics , medicine , physics , biology , ecology , cancer , thermodynamics
Confocal scanning laser microscopy provides the opportunity to obtain three‐dimensional (3‐D) images by piling up consecutive confocal planes. This technique was applied to capture 3‐D images from 100‐μm‐thick tissue blocks from prostate lesions (hyperplasia, dysplasia, adenocarcinomas). Automated methods were implemented to perform a nuclear grading of 3‐D cell nuclei from these specimens. Special attention was focused on the development of a new approach to 3‐D chromatin texture analysis. This method uses mathematical morphology operations to tessellate the chromatin into homogeneous domains. The nuclear features (volume, shape, texture) were subjected to a discriminant analysis. Using a set of five features, the classification of cell nuclei yielded an accuracy of 963%. The results indicate the potential of 3‐D imaging and analysis techniques for an automated nuclear grading of prostate lesions.