Premium
High‐pressure freezing of tissue obtained by fine‐needle biopsy
Author(s) -
HOHENBERG H.,
TOBLER M.,
MÜLLER M.
Publication year - 1996
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1046/j.1365-2818.1996.820642.x
Subject(s) - biological tissue , tissue sample , biopsy , materials science , biomedical engineering , sizing , high pressure , chemistry , pathology , medicine , mechanics , physics , organic chemistry
High‐pressure freezing (HPF) permits adequate cryoimmobilization (without detectable ice crystals after freeze‐substitution) of biological tissue up to a thickness of about 200 μm. Until now the preparation of tissue prior to freezing has been unsatisfactory: sizing of the tissue to the required dimensions takes minutes, during which structural alterations must occur. We demonstrate that the use of a fine‐needle biopsy technique minimizes tissue damage and guarantees sample dimensions close to the optimal thickness for HPF. The tissue cores can be cryoimmobilized within 40 s of excision.