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Video microscopy of dynamic plant cell organelles: principles of the technique and practical application
Author(s) -
LICHTSCHEIDL I. K.,
FOISSNER I.
Publication year - 1996
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1046/j.1365-2818.1996.105385.x
Subject(s) - video microscopy , microscopy , fluorescence microscope , cytoplasmic streaming , microfilament , organelle , actin , golgi apparatus , differential interference contrast microscopy , live cell imaging , microbiology and biotechnology , confocal microscopy , cytoskeleton , biophysics , chemistry , biology , optics , fluorescence , cell , physics , biochemistry , endoplasmic reticulum
Video microscopy, including video‐enhanced contrast, ultraviolet and video‐intensified fluorescence microscopy, was applied to the visualization and analysis of organelles and cytoskeletal elements at the border of resolution of the light microscope. We describe the principles of video microscopy and the necessary technical equipment, and discuss the advantages and limitations with the example of three selected plant cells. In characean internodal cells we observed the motility and disappearance of Golgi secretory vesicles during wound wall formation by video‐enhanced contrast microscopy. In Byblis gland hairs we investigated the movement of different organelles along bundles of actin microfilaments by ultraviolet microscopy, and in onion inner epidermal cells we visualized the arrangement of actin microfilaments during different stages of plasmolysis with video‐intensified fluorescence microscopy.