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Genome‐based detection methods of Macrobrachium rosenbergii nodavirus, a pathogen of the giant freshwater prawn, Macrobrachium rosenbergii : dot‐blot, in situ hybridization and RT‐PCR
Author(s) -
Sri Widada J,
Durand S,
Cambournac I,
Qian D,
Shi Z,
Dejonghe E,
Richard V,
Bonami J R
Publication year - 2003
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1046/j.1365-2761.2003.00493.x
Subject(s) - macrobrachium rosenbergii , prawn , biology , pathogen , dot blot , macrobrachium , virology , genome , fishery , gene , microbiology and biotechnology , genetics , decapoda , crustacean
The availability of specific and rapid detection methods is essential for monitoring the health status of farmed species, particularly in viral diseases as in this case early diagnosis is a critical factor in containing disease outbreaks. Three complementary genome‐based methods were developed for the detection of Macrobrachium rosenbergii nodavirus ( Mr NV), i.e. dot‐blot hybridization, in situ hybridization and reverse transcriptase‐polymerase chain reaction (RT‐PCR). Detection limits were established for dot‐blot hybridization and RT‐PCR and are c. 7 fg and 8 pg of viral RNA, respectively. In situ hybridization indicated that infection was confined to the striated muscle tissue. As a result of its sensitivity, RT‐PCR can be used for in‐depth investigations to examine the extent of the viral infection and establish the onset of infection in hatcheries. The application of RT‐PCR on samples collected from prawn farms in China showed the possible use of this method in routine health monitoring.