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Experimental Cryptobia salmositica (Kinetoplastida) infections in Atlantic salmon, Salmo salar L.: cell‐mediated and humoral immune responses against the pathogenic and vaccine strains of the parasite
Author(s) -
Ardelli B F,
Woo P T K
Publication year - 2002
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1046/j.1365-2761.2002.00369.x
Subject(s) - biology , salmo , immune system , virology , parasite hosting , microbiology and biotechnology , immunology , ichthyophthirius multifiliis , immunogenicity , fish <actinopterygii> , fishery , world wide web , computer science
Hatchery‐reared Atlantic salmon, Salmo salar L., were vaccinated intraperitoneally (i.p.) with a live attenuated Cryptobia salmositica vaccine (either 100 000 or 5000 parasites fish −1 ) and 4 weeks later were challenged with the parasite (either 100 000 or 5000 parasites fish −1 ). Unvaccinated, infected salmon had high parasitaemias and were anaemic. Fish given a high dose (100 000 parasites fish −1 ) had higher parasitaemias than fish given the lower dose. Vaccinated fish had low parasitaemias and a mild anaemia, but recovered quickly after challenge. Complement‐fixing antibody increased in vaccinated fish after challenge and was highest at 2 weeks post‐challenge. The cell‐mediated response (both T cells and B cells) was depressed in infected fish until 4 weeks after infection. In vaccinated fish, the humoral response (i.e. B‐lymphocytes) was greater than the cell‐mediated response (i.e. T‐lymphocytes). In contrast, infected fish had a greater cell‐mediated than humoral immune response.