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Inhibitory effects of a monoclonal antibody (MAb‐001) on in vitro oxygen consumption and multiplication of the pathogenic haemoflagellate, Cryptobia salmositica Katz
Author(s) -
Hontzeas N,
Feng S,
Woo P T K
Publication year - 2001
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1046/j.1365-2761.2001.00315.x
Subject(s) - monoclonal antibody , biology , cysteine , microbiology and biotechnology , in vitro , parasite hosting , antibody , enzyme , virology , biochemistry , immunology , world wide web , computer science
A monoclonal antibody (MAb‐001), against a surface glycoprotein on Cryptobia salmositica inhibited the multiplication and oxygen consumption of both virulent and avirulent strains of the parasite. The classical cysteine proteinase inhibitor (E‐64) and a cysteine proteinase activator (EDTA) affected the in vitro multiplication of C. salmositica . Concentrations of E‐64 higher than 10 μ M reduced the multiplication of C. salmositica while 5 m M of EDTA enhanced its multiplication. We propose that the cysteine proteinase is an important metabolic enzyme in C. salmositica and that binding of MAb‐001 to the enzyme inhibited parasite multiplication and reduced oxygen consumption.