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Characterisation of Aeromonas strains and species by pulsed field gel electrophoresis and principal components analysis
Author(s) -
Livesley M. A.,
Smith S. N.,
Armstrong R. A.,
Barker G. A.
Publication year - 1999
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1046/j.1365-2761.1999.00186.x
Subject(s) - pulsed field gel electrophoresis , biology , restriction enzyme , aeromonas salmonicida , hindiii , aeromonas , typing , gel electrophoresis , microbiology and biotechnology , aeromonas hydrophila , principal component analysis , restriction fragment length polymorphism , vibrionaceae , ribotyping , restriction fragment , genetics , dna , polymerase chain reaction , bacteria , gene , genotype , artificial intelligence , computer science
Aeromonas genomes were investigated by restriction digesting chromosomal DNA with the endonuclease Xba I, separation of restriction fragments by pulsed field gel electrophoresis (PFGE) and principal components analysis (PCA) of resulting separation patterns. A. salmonicida salmonicida were unique amongst the isolates investigated. Separation profiles of these isolates were similar and all characterised by a distinct absence of bands in the 250kb region. Principal components analysis represented these strains as a clearly defined homogeneous group separated by insignificant Euclidian distances. However, A. salmonicida achromogenes isolates in common with those of A. hydrophila and A. sobria were shown by principal components analysis to be more heterogeneous in nature. Fragments from these isolates were more uniform in size distribution but as demonstrated by the Euclidian distances attained through PCA potentially characteristic of each strain. Furthermore passaging of Aeromonas isolates through an appropriate host did not greatly modify fragment separation profiles, indicative of the genomic stability of test aeromonads and the potential of restriction digesting/PFGE/PCA in Aeromonas typing.