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Optimising of culture conditions and stimulation of head kidney macrophages from Atlantic cod, Gadus morhua L.
Author(s) -
Steiro K.,
Johansen A.,
Gildberg A.,
Bøgwald J.
Publication year - 1998
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1046/j.1365-2761.1998.00110.x
Subject(s) - gadus , atlantic cod , biology , stimulation , trypan blue , fishery , lipopolysaccharide , macrophage , respiratory burst , kidney , fish <actinopterygii> , immunology , cell , biochemistry , endocrinology , in vitro
The cultivation of Atlantic cod, Gadus morhua L., head kidney macrophages was optimal at 4–6 °C. The viability of cells in culture was examined by the trypan blue exclusion test 2, 5 and 7 days after seeding. Simultaneously, the number of adherent cells at the various times was checked. The morphology of the cells was also evaluated during the cultivation period. Enhanced respiratory burst activities were demonstrated in macrophages after stimulation with known immunostimulants like lipopolysaccharide, tuftsin and an acid peptide fraction previously shown to stimulate Atlantic salmon leucocytes efficiently. Generally, the Atlantic cod macrophages seemed to respond to a lesser extent compared with macrophages obtained from both mammalian and other fish species.