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In vitro secretion of metallo‐protease (200 kDa) by the pathogenic piscine haemoflagellate, Cryptobia salmositica Katz, and stimulation of protease production by collagen
Author(s) -
Zuo,
; Woo
Publication year - 1998
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1046/j.1365-2761.1998.00098.x
Subject(s) - protease , biology , secretion , microbiology and biotechnology , in vitro , biochemistry , enzyme
Cryptobia salmositica cultured in minimum essential medium secreted metallo‐protease, and a significantly higher amount of the protease was found in media supplemented with either type I or type IV collagen. The enhancement of the protease secretion by type I collagen was dose‐dependent. Using haemoglobin (substrate) SDS‐PAGE, the secreted protease was detected as a single clear band (about 200 kDa). The 200 kDa metallo‐protease was also detected on the C. salmositica surface membrane and the amount was increased by incubating the parasite with collagen. Collagen as a specific substrate may enhance the role of the C. salmositica metallo‐protease in the disease process in infected fish.