Premium
Genetic similarity of iridoviruses from Japan and Thailand
Author(s) -
Miyata M,
Matsuno K,
Jung S J,
Danayadol Y,
Miyazaki T
Publication year - 1997
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1046/j.1365-2761.1997.d01-115.x
Subject(s) - iridovirus , sea bass , biology , polymerase chain reaction , fishery , genetics , fish <actinopterygii> , gene
Iridovirus infection causes serious economic damage in marine cultured fish in Japan, Hong Kong and Singapore, and the incidence of this disease has been increasing. Iridovirus of sea bass from offshore Hong Kong was isolated to determine the genetic similarities of the causative agents. The genomic DNA of iridovirus was purified and cloned. Four DNA clones were randomly chosen and sequenced to generate primers for the polymerase chain reaction (PCR). Corresponding DNA fragments of iridoviruses from sea bass from offshore Hong Kong, red sea bream in Japan and grouper in Thailand were detected. The analogous PCR products from geographically diverse iridoviruses may indicate a widespread distribution of an iridovirus of a single origin.