Premium
Absorption of immunomodulating β(1,3)‐glucan in yolk sac larvae of Atlantic halibut, Hippoglossus hippoglossus (L.)
Author(s) -
Strand H.K.,
Dalmo R.A.
Publication year - 1997
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1046/j.1365-2761.1997.d01-107.x
Subject(s) - halibut , hippoglossus hippoglossus , biology , yolk sac , larva , fishery , anatomy , andrology , botany , fish <actinopterygii> , embryo , medicine
A soluble immunomodulating β(1,3)‐glucan, laminaran, was given in drinking water to yolk‐sac larvae of Atlantic halibut, Hippoglossus hippoglossus L. The larvae absorbed laminaran when the concentration was 25 mg l –1 . After 5 days exposure to laminaran, each larva contained 2.3 ng and 46 ng of 3 H‐ and 125 I‐labelled laminaran, respectively. After 10 days exposure, each larva contained 4.5 ng and 47 ng of 3 H‐ and 125 I‐laminaran, respectively. The absorption was confirmed by fluorescence microscopy of larvae exposed to fluorescein‐labelled laminaran (FITC‐laminaran). The fluorescence was localized to cells in the intestinal epithelial layer and the skin epithelial layer.