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Survival and humoral antibody response of Atlantic salmon, Salmo salar L., vaccinated against Aeromonas salmonicida ssp. achromogenes
Author(s) -
Gudmundsdóttir B K,
Jónsdóttir H,
Steinthórsdóttir V,
Magnadóttir B,
Gudmundsdóttir S
Publication year - 1997
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1046/j.1365-2761.1997.00308.x
Subject(s) - aeromonas salmonicida , salmo , vaccination , biology , antibody , microbiology and biotechnology , immune system , antigen , humoral immunity , antibody titer , virology , immunology , titer , fish <actinopterygii> , fishery
Atlantic salmon were vaccinated against Aeromonas salmonicida ssp. achromogenes (Asa) by injection with three vaccines developed in our laboratory and an autogenous bacterin (IcelandBiojec.OO, IBOO) produced by a commercial vaccine producer. The humoral antibody responses to bacterial antigens were monitored by ELISA and Western blotting. The fish were challenged by infection with Asa 6 and 12 weeks post‐vaccination. Protection was induced in all groups of vaccinated fish. The protection achieved was time‐dependent. The autogenous bacterin, IBOO, induced a protective immune response later than our experimental vaccines. All the vaccines tested induced specific antibody response that increased between 6 and 12 weeks after vaccination. The antibody response was mainly directed against the A‐layer protein, but antibodies to other bacterial components were also detected. Significant correlation was obtained between the antibody titre to extracellular Asa antigens, induced by the different vaccine preparations, and survival of vaccinated fish challenged by a virulent Asa strain. Furthermore, the detection of antibodies directed against an extracellular toxic metallo‐caseinase, AsaP1, in fish sera correlated with protection.