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A vital staining technique with fluorescein diacetate (FDA) and propidium iodide (PI) for the determination of viability of myxosporean and actinosporean spores
Author(s) -
Yokoyama H.,
Danjo T.,
Ogawa K.,
Wakabayashi H.
Publication year - 1997
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1046/j.1365-2761.1997.00293.x
Subject(s) - spore , propidium iodide , biology , staining , microbiology and biotechnology , vital stain , fluorescein , fluorescence , biochemistry , apoptosis , genetics , programmed cell death , physics , quantum mechanics
A vital staining technique with fluorescein diacetate (FDA) and propidium iodide (PI) was used for determination of viability of myxosporean stage spores of Myxobolus artus and actinosporean stage spores of M. cultus . Viable spores stained green with FDA and non‐viable spores stained red with PI were clearly distinguishable in M. artus myxosporean spores released from live infected fish, while the spores collected from pseudocysts were not well stained with FDA. Immediately after release from the oligochaete, Branchiura sowerbyi , actinosporean spores of M. cultus , stained bright green in the sporoplasms and red in the spore processes. In vitro survivability tests revealed that the longevity of M. artus spores was 5 months at 25°C and 15 months at 18°C. Drying and ultraviolet irradiation (36 000 mW s cm –2 for M. artus myxosporean spores, 600 mW s cm –2 for M. cultus actinosporean spores) were most effective as sporicidal treatments.