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Characterization of rainbow trout, Oncorhynchus mykiss (Walbaum), serum creatine kinase isoenzymes and isoforms by means of electrophoresis and isoelectric focusing
Author(s) -
Williams J,
Stewart P,
Timpson P
Publication year - 1996
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1046/j.1365-2761.1996.d01-96.x
Subject(s) - rainbow trout , isoelectric focusing , isozyme , gene isoform , creatine kinase , biology , isoelectric point , agarose , polyacrylamide gel electrophoresis , agarose gel electrophoresis , trout , microbiology and biotechnology , electrophoresis , gel electrophoresis , biochemistry , enzyme , fish <actinopterygii> , fishery , gene
Serum samples were obtained from rainbow trout, Oncorhynchus mykiss (Walbaum), from a commercial fish farm. Creatine kinase (CK; EC 2.7.3.2) isoenzymes and sub‐bands (isoforms) were studied using agarose gel electrophoresis and polyacrylamide gel isoelectric focusing. Creatine kinase isoenzymes and isoforms (sub‐bands) were found to display disparate electrophoretic mobility and isoelectric points, respectively, when compared with CK patterns of human origin. The CK‐BB isoenzyme, rarely detected in human serum, was detected in all trout samples assayed. Up to eight CKMM isoforms were detected by the polyacrylamide gel IEF method described.