Premium
Identification and elimination of mycoplasmas in fish cell line cultures
Author(s) -
Frerichs G N
Publication year - 1996
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1046/j.1365-2761.1996.00258.x
Subject(s) - biology , mycoplasma , microbiology and biotechnology , fish <actinopterygii> , cell culture , ciprofloxacin , staining , virology , antibiotics , genetics , fishery
Mycoplasma contamination was identified in 11 cell lines derived from both coldwater and warmwater fish using DNA staining, microbiological culture and ELISA test detection methods. Acholeplasma laidlawii and Mycoplasma arginini were present as single or combined infections. Treatment of cells over three to six serial subcultivations with the addition of 0.5 μg m −1 mycoplasma removal agent (MRA) or 10 μg m −1 ciprofloxacin to culture growth medium effectively eliminated all contaminants except for one instance of M. arginini infection.