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Facile and rapid high‐performance liquid chromatography method for simultaneous determination of allopurinol and oxypurinol in human serum
Author(s) -
Tada H.,
Fujisaki A.,
Itoh K.,
Suzuki T.
Publication year - 2003
Publication title -
journal of clinical pharmacy and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.622
H-Index - 73
eISSN - 1365-2710
pISSN - 0269-4727
DOI - 10.1046/j.1365-2710.2003.00488.x
Subject(s) - allopurinol , chromatography , protein precipitation , chemistry , high performance liquid chromatography , coefficient of variation , therapeutic drug monitoring , therapeutic index , quantitative analysis (chemistry) , pharmacology , drug , medicine , pathology
Summary Objective: To develop a rapid and sensitive assay for the simultaneous determination of allopurinol and oxypurinol in serum. Method: High‐performance liquid chromatography (HPLC) with UV‐detection. Sample preparation consists of protein precipitation by an addition of trichloracetic acid. Results: Percentage recovery and intra‐assay coefficient of variation (CV%) for allopurinol were 97·4–101·3 and 0·66–5·13, respectively, in the concentration range 0·5–5·0 μg/mL. For oxypurinol, the percentage recovery and the intra‐assay CV% were 93·2–98·1 and 0·88–5·62, respectively, in the concentration ranges 0·4–20 μg/mL. There was no interference of endogenous compounds in this assay. Conclusion: This method is useful for routine therapeutic drug monitoring of allopurinol in a clinical setting.