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Structure–activity relationship: analyses of p‐glycoprotein substrates and inhibitors
Author(s) -
Wang R. B.,
Kuo C. L.,
Lien L. L.,
Lien E. J.
Publication year - 2003
Publication title -
journal of clinical pharmacy and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.622
H-Index - 73
eISSN - 1365-2710
pISSN - 0269-4727
DOI - 10.1046/j.1365-2710.2003.00487.x
Subject(s) - lipophilicity , quantitative structure–activity relationship , chemistry , molecular descriptor , in vivo , molecule , computational chemistry , stereochemistry , organic chemistry , biology , microbiology and biotechnology
Summary Objective: A large number of structurally and functionally diverse compounds act as substrates or modulators of p‐glycoprotein (p‐gp). Some of them possess multiple drug resistance (MDR)‐reversing activity, but only a small number of them have entered clinical study. In order to uncover the factors which exert a significant impact on the interaction between substrates/modulators and p‐gp, we have performed structure–activity relationship (SAR) analyses, including molecular modelling, two‐dimensional (2D) and three‐dimensional (3D) parameter‐frame‐setting analysis, quantitative structure activity relationship (QSAR) analysis among substrates/modulators, as well as clinically promising MDR‐reversing agents. Methods: The physicochemical parameters C log P , CMR and all regression equations were derived by using C log P version 4·0 and the latest CQSAR software, respectively. Molecular modelling and all other parameter calculations were performed by using HyperChem version 5·0 program, after geometry optimization and energy minimization using the AM 1 semiempirical method. Results: SAR analyses indicate that MDR reversal activity is correlated with the lipophilicity ( C log P ), molecular weight (log M w ), longest chain ( N lc ) of the molecule and the energy of the highest occupied orbital ( E homo ). In addition, the presence of a basic tertiary nitrogen atom in the structure is also an important contributor to p‐gp inhibitory activity. Some separation in space is achieved for different subsets of p‐gp substrates and inhibitors using N lc , C log P and E homo as three independent parameters in the 3D‐parameter‐frame setting. Conclusion: A highly effective p‐gp modulator candidate should possess a log P value of 2·92 or higher, 18‐atom‐long or longer molecular axis, and a high E homo value, as well as at least one tertiary basic nitrogen atom. The results obtained may be useful in explaining drug–p‐gp interactions for different compounds, including drug interactions and the development of new MDR chemosensitizers.