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Voltammetric determination of nitrosocimetidine in simulated gastric juice
Author(s) -
Walash M. I.,
Belal F.,
Ibrahim F.,
Hefnawy M.,
Eid M.
Publication year - 2002
Publication title -
journal of clinical pharmacy and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.622
H-Index - 73
eISSN - 1365-2710
pISSN - 0269-4727
DOI - 10.1046/j.1365-2710.2002.00432.x
Subject(s) - polarography , dropping mercury electrode , chemistry , cimetidine , peak current , electrode , analytical chemistry (journal) , chromatography , nitroso , electrochemistry , inorganic chemistry , medicine , organic chemistry
Summary Objective: To develop a voltammetric method for the determination of nitrosocimetidine in simulated gastric juice (SGJ). Method: Measurement of the polarographic wave of the nitroso derivative at the dropping mercury electrode in 0·1 m HCl. Results: A well‐defined diffusion‐controlled cathodic wave was obtained at −0·34 V vs. Ag/AgCl electrode. The current–concentration relationship was found to be rectilinear over the range 10–80 μ g/mL and 2–50 μ g/mL with percentage recovery of 101·24 ± 0·79 and 100·79 ± 0·56 for the direct current (DC t ) and differential pulse polarography (DPP) modes, respectively, with a minimum detectability ( S / N =2) of 0·16 μ g/ml (5·6 × 10 −7 m ) using DPP mode. Conclusion: The proposed method was successfully applied to study the nitrosation of cimetidine in SGJ.