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Microanalysis of propofol in human serum by semi‐microcolumn high‐performance liquid chromatography with UV detection and solid‐phase extraction
Author(s) -
Teshima D.,
Nagahama H.,
Makino K.,
Kataoka Y.,
Oishi R.
Publication year - 2001
Publication title -
journal of clinical pharmacy and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.622
H-Index - 73
eISSN - 1365-2710
pISSN - 0269-4727
DOI - 10.1046/j.1365-2710.2001.00375.x
Subject(s) - chromatography , solid phase extraction , detection limit , high performance liquid chromatography , propofol , cartridge , extraction (chemistry) , elution , sedation , chemistry , materials science , anesthesia , medicine , metallurgy
Objective: To develop a simple analytical method for monitoring the low serum levels of propofol found when administered for the sedation of patients in the intensive care unit (ICU). Methods: A high‐performance liquid chromatographic method (HPLC) was used with UV detection. Solid‐phase extraction (SPE) cartridges and a semi‐microcolumn (TSK gel ODS‐80Ts, 2·0 mm i.d. × 25 cm, 5 μm) were used to improve sensitivity. Propofol in the eluate obtained from the SPE cartridge was concentrated to about five times the initial concentration. Results: The sensitivity using the semi‐microcolumn was amplified by about three‐fold. The assay showed a good linearity with a quantification limit 20 ng/mL. Intra‐ and inter‐assay coefficients of variation were less than 2·2% and 10·0%, respectively. The mean recoveries ranged from 97·6 to 109·5%. Conclusion: The HPLC method described should be useful for measuring the low serum propofol levels found when the drug is used for ICU sedation.