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The effects of gentamicin and vitamin E on enzymatic antioxidant defence in guinea‐pig lung
Author(s) -
Öztürk H. S,
Kavutcu M,
Canbolat O,
Kaçmaz M,
Hadi Yaşa M,
Durak I
Publication year - 1997
Publication title -
journal of clinical pharmacy and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.622
H-Index - 73
eISSN - 1365-2710
pISSN - 0269-4727
DOI - 10.1046/j.1365-2710.1997.00122.x
Subject(s) - superoxide dismutase , antioxidant , catalase , glutathione peroxidase , xanthine oxidase , chemistry , vitamin e , gentamicin , enzyme , pharmacology , biochemistry , glutathione , lung , medicine , biology , antibiotics
Objective:To study the possible effects of gentamicin on the enzymic free‐radical defence system in the lung. Method: Activities of cytoplasmic superoxide dismutase (CuZn‐SOD), mitochondrial superoxide dismutase (Mn‐SOD), glutathione peroxidase (GSH‐Px) and catalase (CAT) enzymes were studied in lung tissues from gentamicin‐treated guinea‐pigs compared to controls. Results: Levels of those enzymes were higher in the gentamicin group except for xanthine oxidase (XO) activity. Vitamin E given concomitantly with gentamicin caused significant decreases in CuZn‐SOD, Mn‐SOD and GSH‐Px activities but an increase in CAT activity in the lung tissue. Only vitamin treatment caused significant decreases in the activities of CuZn‐SOD, Mn‐SOD and GSH‐Px enzymes and an increase in CAT activity. Conclusion: The results suggest that lung tissue is able to respond quickly and effectively against the adverse effects of some oxidant substances by inducing and/or activating the enzymatic free‐radical defence system.