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Characterization of a mosquitocidal Bacillus thuringiensis serovar sotto strain isolated from Okinawa, Japan
Author(s) -
Ohgushi A.,
Wasano N.,
Shisa N.,
Saitoh H.,
Mizuki E.,
Maeda M.,
Ohba M.
Publication year - 2003
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1046/j.1365-2672.2003.02068.x
Subject(s) - bacillus thuringiensis , microbiology and biotechnology , strain (injury) , serotype , bacillales , biology , toxin , bacteria , bacillaceae , bacillus subtilis , genetics , anatomy
Aims: To characterize the mosquitocidal activity of parasporal inclusions of the Bacillus thuringiensis serovar sotto strain 96‐OK‐85‐24, for comparison with two well‐characterized mosquitocidal strains. Methods and Results: The strain 96‐OK‐85‐24 significantly differed from the existing mosquitocidal B. thuringiensis strains in: (1) lacking the larvicidal activity against Culex pipiens molestus and haemolytic activity, and (2) SDS–PAGE profiles, immunological properties and N‐terminal amino acid sequences of parasporal inclusion proteins. Conclusions: It is clear from the results that the strain 96‐OK‐85‐24 synthesizes a novel mosquitocidal Cry protein with a unique toxicity spectrum. Significance and Impact of the Study: This is the first report of the occurrence of a mosquitocidal B. thuringiensis strain with an unusual toxicity spectrum, lacking the activity against the culicine mosquito.