Premium
Correlation between bacterial haemoglobin gene ( vgb ) and aeration: their effect on the growth and α ‐amylase activity in transformed Enterobacter aerogenes
Author(s) -
Khleifat K.,
Abboud M. M.
Publication year - 2003
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1046/j.1365-2672.2003.01939.x
Subject(s) - enterobacter aerogenes , amylase , gene , microbiology and biotechnology , library science , biology , enzyme , genetics , computer science , biochemistry , escherichia coli
Aims: To evaluate the effects of bacterial haemoglobin on bacterial growth and α ‐amylase formation under different aeration conditions. Methods and Results: Enterobacter aerogenes was transformed with the gene encoding Vitreoscilla (bacterial) haemoglobin, vgb . The growth kinetics and ability to synthesize α ‐amylase enzyme were investigated in this transformed Enterobacter strain as well as in two other Enterobacter control strains that do not harbour the vgb gene. Such comparison was made under variable aeration conditions, using the agitation rate as a measure of aeration. The expression of bacterial haemoglobin‐supported cell growth determined as O.D. 600 and cell viability in addition to the α ‐amylase production. These positive effects of bacterial haemoglobin were observed under both low and high aerations, but at different extents. Conclusions: In addition to improving cell growth under low aeration, the bacterial haemoglobin is able to promote bacterial cell tolerance during exposure to high oxygen tension. Significance and Impact of the Study: The expression of bacterial haemoglobin is advantageous in reducing the burden of certain toxic conditions such as high oxygen levels. It may have the same impact on some environmental toxic substances. This, haemoglobin biotechnology can be extended to induce enzymes of pollutants degradation or production of some useful industrial substances.