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Development of a 5′‐nuclease PCR assay for detecting Shiga toxin‐producing Escherichia coli O145 based on the identification of an ‘O‐island 29’ homologue
Author(s) -
Perelle S.,
Dilasser F.,
Grout J.,
Fach P.
Publication year - 2003
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1046/j.1365-2672.2003.01872.x
Subject(s) - escherichia coli , nuclease , biology , polymerase chain reaction , serotype , dna , microbiology and biotechnology , dna sequencing , genetics , gene
Aims: A DNA sequence, from Escherichia coli STEC O145, homologous to O‐island 29 from STEC O157 is described, together with a real‐time PCR assay for detecting it. Methods and Results: PCR and sequencing were used to identify the ‘O‐island 29’ homologous DNA sequence from STEC O145 (strain VTH34). The sequence divergence between the STEC O145 and O157 ‘O‐island 29’ allowed a STEC O145 5′‐nuclease PCR assay to be developed. Conclusions: The characterization of a novel locus in STEC O145 has allowed a specific O145 serogroup 5′‐nuclease PCR assay to be designed. Significance and Impact of the Study: These findings increase the number of serogroup PCR assays available as alternatives to classical O‐serotyping of E. coli .