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The response of Leuconostoc mesenteroides to low external oxidoreduction potential generated by hydrogen gas
Author(s) -
Bourel G.,
Henini S.,
Diviès C.,
Garmyn D.
Publication year - 2003
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1046/j.1365-2672.2003.01832.x
Subject(s) - leuconostoc mesenteroides , leuconostoc , food science , chemistry , hydrogen , biochemistry , biology , bacteria , lactic acid , organic chemistry , fermentation , genetics , lactobacillus
Aims: The physiological consequences of low external oxidoreduction potential in Leuconostoc mesenteroides were investigated. Methods and Results: Leuconostoc mesenteroides was grown under two initial oxidoreduction potential conditions ( E h7 : +200 mV and −400 mV) using nitrogen and hydrogen as reducing agents. Growth was affected by E h7 ; the lag phase increased from 1 h at an initial E h7 of +200 mV to 6 h at an initial E h7 of −400 mV; the maximum specific growth rate at −400 mV was 68% of the one observed at +200 mV. The NADH/NAD + ratio and (NADH + NAD + ) pool were independent of the external E h7 . Conclusions: This study shows that changing the external oxidoreduction potential from +200 to −400 mV has a strong effect on the Leuc. mesenteroides physiology. The constancy of the maximum carbon and energetic fluxes ( q glu , q ATP ) under the two E h7 conditions accompanied by the decrease of Y X / S and Y ATP suggested the existence of an uncoupling phenomenon, namely that some catabolized glucose and hence ATP was not associated with biomass production. Significance and Impact of the Study: This paper demonstrates the usefulness of taking into account, the effect of the oxidoreduction potential on the growth of Leuc. mesenteroides in the fermentation process.