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Variability and functional role of chromosomal sequences in 16SrI‐B subgroup phytoplasmas including aster yellows and related strains
Author(s) -
Botti S.,
Bertaccini A.
Publication year - 2003
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1046/j.1365-2672.2003.01809.x
Subject(s) - biology , amplicon , genetics , phytoplasma , restriction fragment length polymorphism , aster yellows , coding region , ribosomal rna , polymorphism (computer science) , ribosomal dna , sequence analysis , gene , polymerase chain reaction , genotype , phylogenetics
Aims : Partial genetic characterization of several chromosomal regions on 35 16SrI‐B phytoplasma strains maintained in periwinkle and collected in different geographical areas from plants of diverse species. Methods and Results : Genes coding for ribosomal protein rpL22, elongation factor EF‐Tu and random cloned sequences amplified with primers AY19p/m, G35p/m and BB88F1/R1 after RFLP analyses showed a high degree of polymorphism among the strains studied. The ribosomal protein (rp) subgroups B and K, and an undescribed subgroup designated N, were identified. Amplicons obtained with primers AY19p/m and BB88F1/R1, revealed a high and a low degree of polymorphism, respectively. Conclusions : A probable spacer role could be attributed to the AY19p/m sequence and a possible coding function to the BB88F1/R1 sequence. No relationship was found among genetic polymorphisms, identified by statistical analyses, and epidemiological or biological parameters. Significance and Impact of the Study : The analyses of five different genomic sequences of the 35 strains belonging to subgroup 16SrI‐B allowed a finer distinction among them, confirming that the polymorphism level of 16S rDNA is too low to be adopted as unique parameter for classification.