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Identification of the O‐antigen biosynthesis genes of Escherichia coli O91 and development of a O91 PCR serotyping test
Author(s) -
Perelle S.,
Dilasser F.,
Grout J.,
Fach P.
Publication year - 2002
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1046/j.1365-2672.2002.01743.x
Subject(s) - serotype , escherichia coli , biology , antigen , gene , locus (genetics) , gene cluster , polymerase chain reaction , microbiology and biotechnology , enterobacteriaceae , genetics
Aims: The aims of the study were to characterize the O91 O‐antigen gene cluster from Shiga toxin‐producing Escherichia coli (STEC) O91 and to provide the basis for a specific PCR test for rapid detection of E. coli O91. Methods and Results: The published primers complementary to JUMPstart and gnd gene, the conserved flanking sequences of O‐antigen genes clusters in E. coli and related species were used to amplify the 10‐kbp O91 O‐antigen biosynthesis locus of STEC O91. A DNA library representative of this cluster allowed two O91 specific probes to be identified, and two specific PCR O91 serotyping tests to be successfully developed. Conclusions: These results confirm that the O‐antigen gene cluster sequences of E. coli allow rapidly a specific O‐antigen PCR assay to be designed. Significance and Impact of the Study: These findings increase the number of PCR‐assays available to replace the classical O‐serotyping among E. coli O‐antigen.